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Anti-Phospho-STAT5-Y694 Antibody

144-50815
RayBiotech
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSTAT5A
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-Phospho-STAT5-Y694 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Conjugate
    Unconjugated
  • Gene ID6776
  • Target name
    STAT5A
  • Target description
    signal transducer and activator of transcription 5A
  • Target synonyms
    MGF, STAT5, signal transducer and activator of transcription 5A, epididymis secretory sperm binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP42229
  • Protein Name
    Signal transducer and activator of transcription 5A
  • Scientific Description
    Phospho-STAT5-Y694 pAb
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of STAT5A/B using anti-STAT5A/B antibody (A01087-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT5A/B antigen affinity purified polyclonal antibody (Catalog # A01087-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT5A/B at approximately 90-95 kDa. The expected band size for STAT5A/B is at 91 kDa.
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