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Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Anti-PIK3R4 Antibody

HPA036032
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPIK3R4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PIK3R4 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID30849
  • Target name
    PIK3R4
  • Target description
    phosphoinositide-3-kinase regulatory subunit 4
  • Target synonyms
    VPS15, p150, phosphoinositide 3-kinase regulatory subunit 4, PI3-kinase p150 subunit, PI3-kinase regulatory subunit 4, phosphatidylinositol 3-kinase-associated p150, phosphoinositide 3-kinase adaptor protein, phosphoinositide-3-kinase, regulatory subunit 4, p150
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ99570
  • Protein Name
    Phosphoinositide 3-kinase regulatory subunit 4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of VPS15/PIK3R4 using anti-VPS15/PIK3R4 antibody (A06618-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VPS15/PIK3R4 antigen affinity purified polyclonal antibody (Catalog # A06618-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VPS15/PIK3R4 at approximately 150 kDa. The expected band size for VPS15/PIK3R4 is at 153 kDa.
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