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Anti-PPP1R13B Antibody

Anti-PPP1R13B Antibody

A28927
Antibodies.com
ApplicationsWestern Blot
Product group Antibodies
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Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-PPP1R13B Antibody
  • Delivery Days Customer
    7
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    >95%
  • Host
    Rabbit
  • Scientific Description
    Rabbit polyclonal antibody to PPP1R13B
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PPP1R13B using anti-PPP1R13B antibody (A07257-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP1R13B antigen affinity purified polyclonal antibody (Catalog # A07257-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP1R13B at approximately 150 kDa. The expected band size for PPP1R13B is at 120 kDa.
Product group Antibodies
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Bethyl Laboratories
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LifeSpan BioSciences
PPP1R13B AntibodyLS-C750380
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References
Bioss Antibodies
ASPP1 Polyclonal AntibodyBS-1282R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetPPP1R13B
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Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
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Atlas Antibodies
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WB analysis of HepG2 cells using GTX46274 PPP1R13B antibody at 0.2-1microg/ml.
Product group Antibodies
GeneTex
ASPP1 antibody, InternalGTX46274
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TargetPPP1R13B
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The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA071472(PPP1R13B Antibody) at dilution 1/35, on the right is treated with synthetic peptide. (Original magnification: x200)
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PPP1R13B AntibodyCSB-PA071472
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