More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/ASPP1 antibody, Internal
WB analysis of HepG2 cells using GTX46274 PPP1R13B antibody at 0.2-1microg/ml.
WB analysis of HepG2 cells using GTX46274 PPP1R13B antibody at 0.2-1microg/ml.
WB analysis of HepG2 cells using GTX46274 PPP1R13B antibody at 0.2-1microg/ml.

ASPP1 antibody, Internal

GTX46274
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetPPP1R13B
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    ASPP1 antibody, Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.2-2.5 ug/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID23368
  • Target name
    PPP1R13B
  • Target description
    protein phosphatase 1 regulatory subunit 13B
  • Target synonyms
    apoptosis-stimulating of p53 protein 1; apoptosis-stimulating protein of p53, 1; ASPP1; p53BP2-like; p85; protein phosphatase 1, regulatory (inhibitor) subunit 13B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96KQ4
  • Protein Name
    Apoptosis-stimulating of p53 protein 1
  • Scientific Description
    This gene encodes a member of the ASPP (apoptosis-stimulating protein of p53) family of p53 interacting proteins. The protein contains four ankyrin repeats and an SH3 domain involved in protein-protein interactions. ASPP proteins are required for the induction of apoptosis by p53-family proteins. They promote DNA binding and transactivation of p53-family proteins on the promoters of proapoptotic genes. Expression of this gene is regulated by the E2F transcription factor. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
References
Bioss Antibodies
ASPP1 Polyclonal AntibodyBS-1282R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetPPP1R13B
  • SizePrice
Western blot using GeneTexs affinity purified anti-ASPP1 to detect over-expressed ASPP1 in MCF-7 cells (lane 2, arrowhead). Lane 1 is a non-transfected control. Lane 3 is MCF-7 cells over-expressing ASPP2. Cell extracts were electrophoresed and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:1,000 dilution. The identity of the lower MW band at approximately 50kDa is unknown. Primary experimental data indicate that the unknown band intensifies in extracts from p53 siRNA knockdown cells.
Product group Antibodies
GeneTex
ASPP1 antibodyGTX48719
ApplicationsWestern Blot, ELISA
TargetPPP1R13B
  • SizePrice
Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Product group Antibodies
Atlas Antibodies
Anti-PPP1R13B AntibodyHPA006394
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetPPP1R13B
  • SizePrice
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA071472(PPP1R13B Antibody) at dilution 1/35, on the right is treated with synthetic peptide. (Original magnification: x200)
Product group Antibodies
Cusabio
PPP1R13B AntibodyCSB-PA071472
ApplicationsELISA, ImmunoHistoChemistry
TargetPPP1R13B
  • SizePrice
Product group Antibodies
RayBiotech
Anti-PPP1R13B (Center) Antibody102-22630
ApplicationsWestern Blot
TargetPPP1R13B
  • SizePrice
Product group Antibodies
Bethyl Laboratories
Rabbit anti-ASPP1 Antibody, Affinity PurifiedA300-917A
ApplicationsImmunoPrecipitation
TargetPPP1R13B
  • SizePrice
Figure 1. Western blot analysis of PPP1R13B using anti-PPP1R13B antibody (A07257-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP1R13B antigen affinity purified polyclonal antibody (Catalog # A07257-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP1R13B at approximately 150 kDa. The expected band size for PPP1R13B is at 120 kDa.
Product group Antibodies
Boster Bio
Anti-PPP1R13B Antibody Picoband(r)A07257-2-CARRIER-FREE
ApplicationsWestern Blot, ELISA
TargetPPP1R13B
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
PPP1R13B AntibodyLS-C750380
ApplicationsWestern Blot
TargetPPP1R13B
  • SizePrice
Anti-PPP1R13B Antibody
Product group Antibodies
Antibodies.com
Anti-PPP1R13B AntibodyA28927
ApplicationsWestern Blot
  • SizePrice