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The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA071472(PPP1R13B Antibody) at dilution 1/35, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA071472(PPP1R13B Antibody) at dilution 1/35, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA071472(PPP1R13B Antibody) at dilution 1/35, on the right is treated with synthetic peptide. (Original magnification: x200)

PPP1R13B Antibody

CSB-PA071472
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetPPP1R13B
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Overview

  • Supplier
    Cusabio
  • Product Name
    PPP1R13B Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23368
  • Target name
    PPP1R13B
  • Target description
    protein phosphatase 1 regulatory subunit 13B
  • Target synonyms
    apoptosis-stimulating of p53 protein 1; apoptosis-stimulating protein of p53, 1; ASPP1; p53BP2-like; p85; protein phosphatase 1, regulatory (inhibitor) subunit 13B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96KQ4
  • Protein Name
    Apoptosis-stimulating of p53 protein 1
  • Scientific Description
    This gene encodes a member of the ASPP (apoptosis-stimulating protein of p53) family of p53 interacting proteins. The protein contains four ankyrin repeats and an SH3 domain involved in protein-protein interactions. ASPP proteins are required for the induction of apoptosis by p53-family proteins. They promote DNA binding and transactivation of p53-family proteins on the promoters of proapoptotic genes. Expression of this gene is regulated by the E2F transcription factor.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PPP1R13B using anti-PPP1R13B antibody (A07257-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP1R13B antigen affinity purified polyclonal antibody (Catalog # A07257-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP1R13B at approximately 150 kDa. The expected band size for PPP1R13B is at 120 kDa.
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References
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