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Immunohistochemical staining of human lung shows very strong granular positivity in cytoplasm in macrophages.
Immunohistochemical staining of human lung shows very strong granular positivity in cytoplasm in macrophages.
Immunohistochemical staining of human lung shows very strong granular positivity in cytoplasm in macrophages.

Anti-PPT1 Antibody

HPA021546
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetPPT1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PPT1
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5538
  • Target name
    PPT1
  • Target description
    palmitoyl-protein thioesterase 1
  • Target synonyms
    ceroid-palmitoyl-palmitoyl-protein thioesterase 1; CLN1; INCL; palmitoyl-protein hydrolase 1; palmitoyl-protein thioesterase 1; PPT
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP50897
  • Protein Name
    Palmitoyl-protein thioesterase 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PPT1 using anti-PPT1 antibody (PB9781). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse liver tissue lysates, Lane 5: HEPG2 whole Cell lysates, Lane 6: 293T whole cell lysates, Lane 7: MCF-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPT1 antigen affinity purified polyclonal antibody (Catalog # PB9781) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPT1.
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GeneTex
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