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Western Blot Positive WB detected in: HEK293 whole cell lysate All lanes: PPT1 antibody at 3.5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 35, 24 kDa Observed band size: 35 kDa
Western Blot Positive WB detected in: HEK293 whole cell lysate All lanes: PPT1 antibody at 3.5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 35, 24 kDa Observed band size: 35 kDa
Western Blot Positive WB detected in: HEK293 whole cell lysate All lanes: PPT1 antibody at 3.5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 35, 24 kDa Observed band size: 35 kDa

PPT1 Antibody

CSB-PA018587LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetPPT1
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Overview

  • Supplier
    Cusabio
  • Product Name
    PPT1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5538
  • Target name
    PPT1
  • Target description
    palmitoyl-protein thioesterase 1
  • Target synonyms
    ceroid-palmitoyl-palmitoyl-protein thioesterase 1; CLN1; INCL; palmitoyl-protein hydrolase 1; palmitoyl-protein thioesterase 1; PPT
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP50897
  • Protein Name
    Palmitoyl-protein thioesterase 1
  • Scientific Description
    Removes thioester-linked fatty acyl groups such as palmitate from modified cysteine residues in proteins or peptides during lysosomal degradation. Prefers acyl chain lengths of 14 to 18 carbons.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PPT1 using anti-PPT1 antibody (PB9781). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse liver tissue lysates, Lane 5: HEPG2 whole Cell lysates, Lane 6: 293T whole cell lysates, Lane 7: MCF-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPT1 antigen affinity purified polyclonal antibody (Catalog # PB9781) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPT1.
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