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Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Anti-PRODH Antibody

HPA020361
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPRODH
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PRODH
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5625
  • Target name
    PRODH
  • Target description
    proline dehydrogenase 1
  • Target synonyms
    HSPOX2; p53-induced gene 6 protein; PIG6; POX; PRODH1; PRODH2; proline dehydrogenase (oxidase) 1; proline dehydrogenase 1, mitochondrial; proline oxidase 2; proline oxidase, mitochondrial; TP53I6; tumor protein p53 inducible protein 6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43272
  • Protein Name
    Proline dehydrogenase 1, mitochondrial
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PRODH using anti-PRODH antibody (A03231-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates, Lane 3: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRODH antigen affinity purified polyclonal antibody (Catalog # A03231-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRODH at approximately 69 kDa. The expected band size for PRODH is at 69 kDa.
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