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Western Blot Positive WB detected in: Rat liver tissue All lanes: PRODH antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 69, 57, 60 kDa Observed band size: 69 kDa
Western Blot Positive WB detected in: Rat liver tissue All lanes: PRODH antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 69, 57, 60 kDa Observed band size: 69 kDa
Western Blot Positive WB detected in: Rat liver tissue All lanes: PRODH antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 69, 57, 60 kDa Observed band size: 69 kDa

PRODH Antibody

CSB-PA018744LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetPRODH
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Overview

  • Supplier
    Cusabio
  • Product Name
    PRODH Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5625
  • Target name
    PRODH
  • Target description
    proline dehydrogenase 1
  • Target synonyms
    HSPOX2, PIG6, POX, PRODH1, TP53I6, proline dehydrogenase 1, mitochondrial, p53 Inducible gene 6, p53-induced gene 6 protein, proline dehydrogenase (oxidase) 1, proline oxidase 2, proline oxidase, mitochondrial, tumor protein p53 inducible protein 6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43272
  • Protein Name
    Proline dehydrogenase 1, mitochondrial
  • Scientific Description
    Converts proline to delta-1-pyrroline-5-carboxylate.
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PRODH using anti-PRODH antibody (A03231-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates, Lane 3: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRODH antigen affinity purified polyclonal antibody (Catalog # A03231-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRODH at approximately 69 kDa. The expected band size for PRODH is at 69 kDa.
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