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Immunofluorescent staining of human cell line REH shows localization to nucleoplasm.
Immunofluorescent staining of human cell line REH shows localization to nucleoplasm.
Immunofluorescent staining of human cell line REH shows localization to nucleoplasm.

Anti-RAG2 Antibody

HPA065704
Atlas Antibodies
ApplicationsImmunoCytoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetRAG2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RAG2
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5897
  • Target name
    RAG2
  • Target description
    recombination activating 2
  • Target synonyms
    RAG-2; recombination activating gene 2; V(D)J recombination-activating protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP55895
  • Protein Name
    V(D)J recombination-activating protein 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Immunofluorescence staining of Hela cells with CSB-PA345128LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Figure 1. Western blot analysis of RAG2 using anti-RAG2 antibody (A00352). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: mouse thymus tissue lysates, Lane 6: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAG2 antigen affinity purified polyclonal antibody (Catalog # A00352) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAG2 at approximately 55 kDa. The expected band size for RAG2 is at 59 kDa.
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