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Immunohistochemistry analysis in human testis and cerebral cortex tissues using HPA027573 antibody. Corresponding RCC1 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human testis and cerebral cortex tissues using HPA027573 antibody. Corresponding RCC1 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human testis and cerebral cortex tissues using HPA027573 antibody. Corresponding RCC1 RNA-seq data are presented for the same tissues.

Anti-RCC1 Antibody

HPA027573
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetRCC1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RCC1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1104
  • Target name
    RCC1
  • Target description
    regulator of chromosome condensation 1
  • Target synonyms
    cell cycle regulatory protein; CHC1; guanine nucleotide-releasing protein; RCC1-I; regulator of chromosome condensation; SNHG3-RCC1; SNHG3-RCC1 readthrough
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP18754
  • Protein Name
    Regulator of chromosome condensation
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RCC1 using anti-RCC1 antibody (A02719-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: human THP-1 whole cell lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse Neuro-2a whole cell lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RCC1 antigen affinity purified polyclonal antibody (Catalog # A02719-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RCC1 at approximately 45KD. The expected band size for RCC1 is at 45KD.
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