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Immunohistochemical staining of human testis shows strong nuclear positivity in spermatocytes and spermatids.
Immunohistochemical staining of human testis shows strong nuclear positivity in spermatocytes and spermatids.
Immunohistochemical staining of human testis shows strong nuclear positivity in spermatocytes and spermatids.

Anti-RCC1 Antibody

HPA027574
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetRCC1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RCC1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1104
  • Target name
    RCC1
  • Target description
    regulator of chromosome condensation 1
  • Target synonyms
    cell cycle regulatory protein; CHC1; guanine nucleotide-releasing protein; RCC1-I; regulator of chromosome condensation; SNHG3-RCC1; SNHG3-RCC1 readthrough
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP18754
  • Protein Name
    Regulator of chromosome condensation
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RCC1 using anti-RCC1 antibody (A02719-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: human THP-1 whole cell lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse Neuro-2a whole cell lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RCC1 antigen affinity purified polyclonal antibody (Catalog # A02719-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RCC1 at approximately 45KD. The expected band size for RCC1 is at 45KD.
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Immunohistochemistry analysis in human testis and cerebral cortex tissues using HPA027573 antibody. Corresponding RCC1 RNA-seq data are presented for the same tissues.
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Product group Antibodies
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