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Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Raji cells, Primary antibody: CSB-PA075110(RCC1 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Raji cells, Primary antibody: CSB-PA075110(RCC1 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Raji cells, Primary antibody: CSB-PA075110(RCC1 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds

RCC1 Antibody

CSB-PA075110
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetRCC1
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Overview

  • Supplier
    Cusabio
  • Product Name
    RCC1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1104
  • Target name
    RCC1
  • Target description
    regulator of chromosome condensation 1
  • Target synonyms
    cell cycle regulatory protein; CHC1; guanine nucleotide-releasing protein; RCC1-I; regulator of chromosome condensation; SNHG3-RCC1; SNHG3-RCC1 readthrough
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP18754
  • Protein Name
    Regulator of chromosome condensation
  • Scientific Description
    RCC1 (regulator of chromosome condensation 1), increases the rate at which Ran exchanges GDP for GTP. Ran GAP1 opposes the effects of RCC1 by increasing the rate at which Ran hydrolyzes GTP to GDP. A protein designated Ran BP1 has no intrinsic GAP activity and functions as a GEF inhibitor, deactivating RCC1 and thereby indirectly increasing the ratio of Ran GDP to Ran GTP. Ran BP2 has been proposed as the Ran GTP docking site at the periphery of the nuclear pore complex.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RCC1 using anti-RCC1 antibody (A02719-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: human THP-1 whole cell lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse Neuro-2a whole cell lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RCC1 antigen affinity purified polyclonal antibody (Catalog # A02719-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RCC1 at approximately 45KD. The expected band size for RCC1 is at 45KD.
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