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Anti-REG1A [A6]

AB04174-1.1
Absolute Antibody
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetREG1A
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-REG1A [A6]
  • Delivery Days Customer
    9
  • Application Supplier Note
    The specificity of the original format of the antibody was confirmed by ELISA analysis. The antibody could specifically bind to the REG1A protein by western blot analysis (CN114395040A).
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    A6
  • Gene ID5967
  • Target name
    REG1A
  • Target description
    regenerating family member 1 alpha
  • Target synonyms
    ICRF; islet cells regeneration factor; islet of langerhans regenerating protein; lithostathine-1-alpha; P19; pancreatic stone protein, secretory; pancreatic thread protein; protein-X; PSP; PSPS; PSPS1; PTP; REG; REG-1-alpha; regenerating islet-derived protein 1-alpha; regenerating protein I alpha
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP05451
  • Protein Name
    Lithostathine-1-alpha
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of REG1A/1B using anti-REG1A/1B antibody (A03075-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat small intestine tissue lysates, Lane 2: mouse small intestine tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-REG1A/1B antigen affinity purified polyclonal antibody (Catalog # A03075-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for REG1A/1B at approximately 19 kDa. The expected band size for REG1A/1B is at 19 kDa.
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