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Anti-REG1A Antibody

CAB5327
Assay Genie
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetREG1A
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Overview

  • Supplier
    Assay Genie
  • Product Name
    Anti-REG1A Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    Not applicable
  • Conjugate
    Unconjugated
  • Gene ID5967
  • Target name
    REG1A
  • Target description
    regenerating family member 1 alpha
  • Target synonyms
    ICRF, P19, PSP, PSPS, PSPS1, PTP, REG, lithostathine-1-alpha, REG-1-alpha, islet cells regeneration factor, islet of langerhans regenerating protein, pancreatic stone protein, secretory, pancreatic thread protein, protein-X, regenerating islet-derived protein 1-alpha, regenerating protein I alpha
  • Host
    Rabbit
  • Protein IDP05451
  • Protein Name
    Lithostathine-1-alpha
  • Scientific Description
    REG1A Antibody is a premium polyclonal antibody that offers outstanding performance and reliability for demanding research applications. Rigorously validated for WB, ELISA, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Human,Mouse,Rat samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 100microL format to meet your experimental needs. For optimal performance, store at -20°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity
    Human
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of REG1A/1B using anti-REG1A/1B antibody (A03075-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat small intestine tissue lysates, Lane 2: mouse small intestine tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-REG1A/1B antigen affinity purified polyclonal antibody (Catalog # A03075-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for REG1A/1B at approximately 19 kDa. The expected band size for REG1A/1B is at 19 kDa.
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Immunohistochemistry analysis in human pancreas and kidney tissues using HPA045549 antibody. Corresponding REG1A RNA-seq data are presented for the same tissues.
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WB analysis of various sample lysates using GTX66464 REG1A antibody. Dilution : 1:1000 Loading : 25μg per lane
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