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WB analysis of various sample lysates using GTX66464 REG1A antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX66464 REG1A antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX66464 REG1A antibody. Dilution : 1:1000 Loading : 25microg per lane

REG1A antibody

GTX66464
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetREG1A
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Overview

  • Supplier
    GeneTex
  • Product Name
    REG1A antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5967
  • Target name
    REG1A
  • Target description
    regenerating family member 1 alpha
  • Target synonyms
    ICRF; islet cells regeneration factor; islet of langerhans regenerating protein; lithostathine-1-alpha; P19; pancreatic stone protein, secretory; pancreatic thread protein; protein-X; PSP; PSPS; PSPS1; PTP; REG; REG-1-alpha; regenerating islet-derived protein 1-alpha; regenerating protein I alpha
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP05451
  • Protein Name
    Lithostathine-1-alpha
  • Scientific Description
    This gene is a type I subclass member of the Reg gene family. The Reg gene family is a multigene family grouped into four subclasses, types I, II, III and IV, based on the primary structures of the encoded proteins. This gene encodes a protein that is secreted by the exocrine pancreas. It is associated with islet cell regeneration and diabetogenesis and may be involved in pancreatic lithogenesis. Reg family members REG1B, REGL, PAP and this gene are tandemly clustered on chromosome 2p12 and may have arisen from the same ancestral gene by gene duplication. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of REG1A/1B using anti-REG1A/1B antibody (A03075-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat small intestine tissue lysates, Lane 2: mouse small intestine tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-REG1A/1B antigen affinity purified polyclonal antibody (Catalog # A03075-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for REG1A/1B at approximately 19 kDa. The expected band size for REG1A/1B is at 19 kDa.
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