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REG1A Antibody

LS-C830298
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetREG1A
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    REG1A Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:5000 - 1:10000), WB (1:500 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5967
  • Target name
    REG1A
  • Target description
    regenerating family member 1 alpha
  • Target synonyms
    ICRF, P19, PSP, PSPS, PSPS1, PTP, REG, lithostathine-1-alpha, REG-1-alpha, islet cells regeneration factor, islet of langerhans regenerating protein, pancreatic stone protein, secretory, pancreatic thread protein, protein-X, regenerating islet-derived protein 1-alpha, regenerating protein I alpha
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of REG1A/1B using anti-REG1A/1B antibody (A03075-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat small intestine tissue lysates, Lane 2: mouse small intestine tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-REG1A/1B antigen affinity purified polyclonal antibody (Catalog # A03075-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for REG1A/1B at approximately 19 kDa. The expected band size for REG1A/1B is at 19 kDa.
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