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Anti-RFA2 Antibody

102-20182
RayBiotech
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetRPA2
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-RFA2 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1591CT183.68.6
  • Gene ID6118
  • Target name
    RPA2
  • Target description
    replication protein A2
  • Target synonyms
    REPA2; replication factor A protein 2; replication protein A 32 kDa subunit; replication protein A 34 kDa subunit; RF-A protein 2; RP-A p32; RP-A p34; RPA32
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP15927
  • Protein Name
    Replication protein A 32 kDa subunit
  • Scientific Description
    Mouse Anti-RFA2 Antibody, 200 microl
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RPA32/RPA2 using anti-RPA32/RPA2 antibody (A02067-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human Raji whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPA32/RPA2 antigen affinity purified polyclonal antibody (Catalog # A02067-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPA32/RPA2 at approximately 29KD. The expected band size for RPA32/RPA2 is at 29KD.
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