More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/RPA2 / RFA2 / RPA34 Antibody

RPA2 / RFA2 / RPA34 Antibody

LS-C770317
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetRPA2
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    RPA2 / RFA2 / RPA34 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA (1:2000 - 1:5000), IHC (1:30 - 1:150), WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.7 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6118
  • Target name
    RPA2
  • Target description
    replication protein A2
  • Target synonyms
    REPA2; replication factor A protein 2; replication protein A 32 kDa subunit; replication protein A 34 kDa subunit; RF-A protein 2; RP-A p32; RP-A p34; RPA32
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Related products

Paraformaldehyde-fixed, paraffin embedded mouse small intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with RPA32 Polyclonal Antibody (bs-4182R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Product group Antibodies
Bioss Antibodies
RPA2 Polyclonal AntibodyBS-4182R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetRPA2
  • SizePrice
Product group Antibodies
Assay Genie
RPA2 AntibodyCAB2189
TargetRPA2
  • SizePrice
Product group Antibodies
Biomatik
RPA2 Polyclonal AntibodyCAC13840
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
TargetRPA2
  • SizePrice
Product group Antibodies
Bethyl Laboratories
Rabbit anti-RPA32 Antibody, Affinity PurifiedA300-244A
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
TargetRPA2
  • SizePrice
Product group Antibodies
MedChem Express
RPA32/RPA2 Antibody (YA679, Mouse)HY-P80975
TargetRPA2
  • SizePrice
Western Blot Positive WB detected in: 293 whole cell lysate All lanes: RPA2 antibody at 2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 30, 31, 39 kDa Observed band size: 30 kDa
Product group Antibodies
Cusabio
RPA2 AntibodyCSB-PA01555A0RB
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetRPA2
  • SizePrice
Immunohistochemical staining of human Testis cancer, carcinoma shows strong nuclear positivity in tumor cells.
Product group Antibodies
Atlas Antibodies
Anti-RPA2 AntibodyHPA026306
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetRPA2
  • SizePrice
Product group Antibodies
RayBiotech
Anti-RFA2 Antibody102-20182
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetRPA2
  • SizePrice
Figure 1. Western blot analysis of RPA32/RPA2 using anti-RPA32/RPA2 antibody (A02067-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human Raji whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPA32/RPA2 antigen affinity purified polyclonal antibody (Catalog # A02067-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPA32/RPA2 at approximately 29KD. The expected band size for RPA32/RPA2 is at 29KD.
Product group Antibodies
Boster Bio
Anti-RPA32/RPA2 Antibody Picoband(r)A02067-1-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetRPA2
  • SizePrice
Product group Antibodies
References
GeneTex
RPA32 antibody [N1C3]GTX101765
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetRPA2
  • SizePrice