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Immunohistochemical staining of human Testis cancer, carcinoma shows strong nuclear positivity in tumor cells.
Immunohistochemical staining of human Testis cancer, carcinoma shows strong nuclear positivity in tumor cells.
Immunohistochemical staining of human Testis cancer, carcinoma shows strong nuclear positivity in tumor cells.

Anti-RPA2 Antibody

HPA026306
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetRPA2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RPA2
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6118
  • Target name
    RPA2
  • Target description
    replication protein A2
  • Target synonyms
    REPA2; replication factor A protein 2; replication protein A 32 kDa subunit; replication protein A 34 kDa subunit; RF-A protein 2; RP-A p32; RP-A p34; RPA32
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP15927
  • Protein Name
    Replication protein A 32 kDa subunit
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: 293 whole cell lysate All lanes: RPA2 antibody at 2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 30, 31, 39 kDa Observed band size: 30 kDa
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Figure 1. Western blot analysis of RPA32/RPA2 using anti-RPA32/RPA2 antibody (A02067-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human Raji whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPA32/RPA2 antigen affinity purified polyclonal antibody (Catalog # A02067-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPA32/RPA2 at approximately 29KD. The expected band size for RPA32/RPA2 is at 29KD.
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