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ProductsBack/RPA32 antibody [N1C3]
Sample (30 ug of whole cell lysate) A: Molt-4 (GTX27912) 12% SDS PAGE GTX101765 diluted at 1:1000
Sample (30 ug of whole cell lysate) A: Molt-4 (GTX27912) 12% SDS PAGE GTX101765 diluted at 1:1000
Sample (30 ug of whole cell lysate) A: Molt-4 (GTX27912) 12% SDS PAGE GTX101765 diluted at 1:1000

RPA32 antibody [N1C3]

GTX101765
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityChicken, Human
TargetRPA2
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Overview

  • Supplier
    GeneTex
  • Product Name
    RPA32 antibody [N1C3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    2 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6118
  • Target name
    RPA2
  • Target description
    replication protein A2
  • Target synonyms
    REPA2, RP-A p32, RP-A p34, RPA32, replication protein A 32 kDa subunit, RF-A protein 2, replication factor A protein 2, replication protein A 34 kDa subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP15927
  • Protein Name
    Replication protein A 32 kDa subunit
  • Scientific Description
    Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
  • Reactivity
    Chicken, Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Nakamura K, Kogame T, Oshiumi H, et al. Collaborative action of Brca1 and CtIP in elimination of covalent modifications from double-strand breaks to facilitate subsequent break repair. PLoS Genet. 2010,6(1):e1000828. doi: 10.1371/journal.pgen.1000828
    Read this paper

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Figure 1. Western blot analysis of RPA32/RPA2 using anti-RPA32/RPA2 antibody (A02067-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human Raji whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPA32/RPA2 antigen affinity purified polyclonal antibody (Catalog # A02067-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPA32/RPA2 at approximately 29KD. The expected band size for RPA32/RPA2 is at 29KD.
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