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Immunohistochemical staining of Ribosomal Protein S3 using anti-Ribosomal Protein S3 (human), mAb (RP159-1) (Prod. No. AG-20A-0048) in human kidney tissue (5microg/ml). This antibody has been tested in immunohistochemistry, analyzed by an anatomic
Immunohistochemical staining of Ribosomal Protein S3 using anti-Ribosomal Protein S3 (human), mAb (RP159-1) (Prod. No. AG-20A-0048) in human kidney tissue (5microg/ml). This antibody has been tested in immunohistochemistry, analyzed by an anatomic
Immunohistochemical staining of Ribosomal Protein S3 using anti-Ribosomal Protein S3 (human), mAb (RP159-1) (Prod. No. AG-20A-0048) in human kidney tissue (5microg/ml). This antibody has been tested in immunohistochemistry, analyzed by an anatomic

anti-Ribosomal Protein S3 (human), mAb (RP159-1)

AG-20A-0048
AdipoGen Life Sciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetRPS3
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Overview

  • Supplier
    AdipoGen Life Sciences
  • Product Name
    anti-Ribosomal Protein S3 (human), mAb (RP159-1)
  • Delivery Days Customer
    10
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    RP159-1
  • Concentration
    1 mg/ml
  • Gene ID6188
  • Target name
    RPS3
  • Target description
    ribosomal protein S3
  • Target synonyms
    40S ribosomal protein S3; IMR-90 ribosomal protein S3; S3; small ribosomal subunit protein uS3
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP23396
  • Protein Name
    40S ribosomal protein S3
  • Scientific Description
    Monoclonal Antibody. Recognizes human ribosomal protein S3. Detects a band of ~32kDa by Western blot. Isotype: Mouse IgG1kappa. Clone: RP159-1. Applications: ELISA, IHC, WB. Liquid. 0.2microm-filtered solution in PBS, pH 7.4. Contains no preservatives. RpS3 is a component of the 40S ribosomal subunit and is an an essential but previously unkown subunit of NF-kappaB involved in the regulation of key genes in rapid cellular activation responses. RpS3 interacts with nm23-H1. The expression of rpS3 reduces the secretion of MMP-9 and the invasive metastatic potential in HT1080 cells. The phosphorylated ERK is reduced by the expression of rpS3. - RpS3 is a component of the 40S ribosomal subunit and is an an essential but previously unkown subunit of NF-kappaB involved in the regulation of key genes in rapid cellular activation responses. RpS3 interacts with nm23-H1. The expression of rpS3 reduces the secretion of MMP-9 and the invasive metastatic potential in HT1080 cells. The phosphorylated ERK is reduced by the expression of rpS3.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RPS3 using anti-RPS3 antibody (A01542-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human HL-60 whole cell lysate, Lane 3: human Caco-2 whole cell lysate, Lane 4: human K562 whole cell lysate, Lane 5: human U2OS whole cell lysate, Lane 6: human MCF-7 whole cell lysate, Lane 7: human PC-3 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS3 antigen affinity purified polyclonal antibody (Catalog # A01542-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPS3 at approximately 31KD. The expected band size for RPS3 is at 27KD.
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