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Anti-RPLP0 Antibody

144-05557
RayBiotech
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetRPLP0
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-RPLP0 Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6175
  • Target name
    RPLP0
  • Target description
    ribosomal protein lateral stalk subunit P0
  • Target synonyms
    L10E, LP0, P0, PRLP0, RPP0, uL10, large ribosomal subunit protein uL10, 60S acidic ribosomal protein P0, 60S ribosomal protein L10E, acidic ribosomal phosphoprotein P0, neutral ribosomal phosphoprotein P0, ribosomal protein, large, P0
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP05388
  • Protein Name
    60S acidic ribosomal protein P0
  • Scientific Description
    RPLP0 Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RPLP0 using anti-RPLP0 antibody (A04349-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human A375 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPLP0 antigen affinity purified polyclonal antibody (Catalog # A04349-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPLP0 at approximately 37 kDa. The expected band size for RPLP0 is at 34 kDa.
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