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Western Blot Positive WB detected in: Rat brain tissue, Rat kidney tissue, Mouse kidney tissue All lanes: RPLP0 antibody at 3.4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 35, 28 kDa Observed band size: 35 kDa
Western Blot Positive WB detected in: Rat brain tissue, Rat kidney tissue, Mouse kidney tissue All lanes: RPLP0 antibody at 3.4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 35, 28 kDa Observed band size: 35 kDa
Western Blot Positive WB detected in: Rat brain tissue, Rat kidney tissue, Mouse kidney tissue All lanes: RPLP0 antibody at 3.4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 35, 28 kDa Observed band size: 35 kDa

RPLP0 Antibody

CSB-PA020336LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetRPLP0
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Overview

  • Supplier
    Cusabio
  • Product Name
    RPLP0 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6175
  • Target name
    RPLP0
  • Target description
    ribosomal protein lateral stalk subunit P0
  • Target synonyms
    60S acidic ribosomal protein P0; 60S ribosomal protein L10E; acidic ribosomal phosphoprotein P0; L10E; large ribosomal subunit protein uL10; LP0; neutral ribosomal phosphoprotein P0; P0; PRLP0; ribosomal protein, large, P0; RPP0
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP05388
  • Protein Name
    60S acidic ribosomal protein P0
  • Scientific Description
    Ribosomal protein P0 is the functional equivalent of E.coli protein L10.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RPLP0 using anti-RPLP0 antibody (A04349-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human A375 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPLP0 antigen affinity purified polyclonal antibody (Catalog # A04349-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPLP0 at approximately 37 kDa. The expected band size for RPLP0 is at 34 kDa.
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