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Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue section using anti-S100B rabbit monoclonal antibody (Clone RM304) at a 1:1000 dilution.
Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue section using anti-S100B rabbit monoclonal antibody (Clone RM304) at a 1:1000 dilution.
Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue section using anti-S100B rabbit monoclonal antibody (Clone RM304) at a 1:1000 dilution.

anti-S100-beta (human), Rabbit Monoclonal (RM304)

REV-31-1189-00
RevMAb Biosciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetS100B
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Overview

  • Supplier
    RevMAb Biosciences
  • Product Name
    anti-S100-beta (human), Rabbit Monoclonal (RM304)
  • Delivery Days Customer
    10
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    RM304
  • Gene ID6285
  • Target name
    S100B
  • Target description
    S100 calcium binding protein B
  • Target synonyms
    NEF; protein S100-B; S100; S100 calcium-binding protein, beta (neural); S-100 calcium-binding protein, beta chain; S-100 protein subunit beta; S100-B; S100beta
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP04271
  • Protein Name
    Protein S100-B
  • Scientific Description
    Recombinant Antibody. This antibody reacts to human S100-B (S-100 protein beta chain). It may also react to mouse or rat S100-B, as predicted by immunogen homology. Applications: WB, IHC. Source: Rabbit. Liquid. 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide. S100-beta (S100B) is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells and are involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100B may function in neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several diseases, including Alzheimers disease, Downs syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma and type I diabetes. - S100-beta (S100B) is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells and are involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100B may function in neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several diseases, including Alzheimers disease, Downs syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma and type I diabetes.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of S100B using anti-S100B antibody (M00979-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat C6 whole cell lysates, Lane 3: mouse brain tissue lysates, After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-S100B antigen affinity purified monoclonal antibody (Catalog # M00979-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for S100B at approximately 11 kDa. The expected band size for S100B is at 11 kDa.
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