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Immunohistochemical staining of human retina shows strong positivity in rod segments.
Immunohistochemical staining of human retina shows strong positivity in rod segments.
Immunohistochemical staining of human retina shows strong positivity in rod segments.

Anti-SAG Antibody

HPA004946
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetSAG
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SAG
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6295
  • Target name
    SAG
  • Target description
    S-antigen visual arrestin
  • Target synonyms
    48 kDa protein; arrestin 1; retinal S-antigen (48 KDa protein); rod arrestin; rod photoreceptor arrestin; RP47; S-AG; S-antigen; retina and pineal gland (arrestin); S-arrestin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP10523
  • Protein Name
    S-arrestin
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Retinal S antigen using anti-Retinal S antigen antibody (A03775). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat eye ball tissue lysates, Lane 2: mouse eye ball tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Retinal S antigen antigen affinity purified polyclonal antibody (Catalog # A03775) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Retinal S antigen at approximately 45-55KD. The expected band size for Retinal S antigen is at 45KD.
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