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ProductsBack/SAG / Arrestin Antibody

SAG / Arrestin Antibody

LS-C748112
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSAG
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SAG / Arrestin Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 45kDa, while the observed MW by Western blot was 48kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:1000 - 1:2000) The predicted MW is 45kDa, while the observed MW by Western blot was 48kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6295
  • Target name
    SAG
  • Target description
    S-antigen visual arrestin
  • Target synonyms
    RP47, RP96, S-AG, S-arrestin, 48 kDa protein, S-antigen; retina and pineal gland (arrestin), arrestin 1, retinal S-antigen (48 KDa protein), rod arrestin, rod photoreceptor arrestin
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Retinal S antigen using anti-Retinal S antigen antibody (A03775). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat eye ball tissue lysates, Lane 2: mouse eye ball tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Retinal S antigen antigen affinity purified polyclonal antibody (Catalog # A03775) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Retinal S antigen at approximately 45-55KD. The expected band size for Retinal S antigen is at 45KD.
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