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Anti-SAP18 Antibody

144-65802
RayBiotech
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetSAP18
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-SAP18 Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Conjugate
    Unconjugated
  • Gene ID10284
  • Target name
    SAP18
  • Target description
    Sin3A associated protein 18
  • Target synonyms
    2HOR0202, SAP18P, histone deacetylase complex subunit SAP18, 18 kDa Sin3-associated polypeptide, Sin3A-associated protein, 18kDa, cell growth inhibiting protein 38, cell growth-inhibiting gene 38 protein, epididymis secretory sperm binding protein, histone deacetlyase complex subunit SAP18, sin3-associated polypeptide, 18 kDa, sin3-associated polypeptide, p18
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00422
  • Protein Name
    Histone deacetylase complex subunit SAP18
  • Scientific Description
    SAP18 pAb
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SAP18 using anti-SAP18 antibody (A06381-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: rat RH35 whole cell lysates, Lane 5: mouse NIN/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAP18 antigen affinity purified polyclonal antibody (Catalog # A06381-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SAP18 at approximately 18 kDa. The expected band size for SAP18 is at 18 kDa.
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