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Immunohistochemical staining of human testis shows nuclear positivity in cells in seminiferous ducts and Leydig cells.
Immunohistochemical staining of human testis shows nuclear positivity in cells in seminiferous ducts and Leydig cells.
Immunohistochemical staining of human testis shows nuclear positivity in cells in seminiferous ducts and Leydig cells.

Anti-SAP18 Antibody

HPA011352
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetSAP18
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SAP18
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10284
  • Target name
    SAP18
  • Target description
    Sin3A associated protein 18
  • Target synonyms
    18 kDa Sin3-associated polypeptide; 2HOR0202; cell growth inhibiting protein 38; cell growth-inhibiting gene 38 protein; epididymis secretory sperm binding protein; histone deacetlyase complex subunit SAP18; histone deacetylase complex subunit SAP18; SAP18P; Sin3A-associated protein, 18kDa; sin3-associated polypeptide, 18 kDa; sin3-associated polypeptide, p18
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00422
  • Protein Name
    Histone deacetylase complex subunit SAP18
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SAP18 using anti-SAP18 antibody (A06381-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: rat RH35 whole cell lysates, Lane 5: mouse NIN/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAP18 antigen affinity purified polyclonal antibody (Catalog # A06381-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SAP18 at approximately 18 kDa. The expected band size for SAP18 is at 18 kDa.
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