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SAP18 antibody detects SAP18 protein by western blot analysis. K562 whole cell extracts and nuclear extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with SAP18 antibody (GTX130522) at a dilution of 1:1000 and developed with Trident femto Western HRP Substrate (GTX14698).
SAP18 antibody detects SAP18 protein by western blot analysis. K562 whole cell extracts and nuclear extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with SAP18 antibody (GTX130522) at a dilution of 1:1000 and developed with Trident femto Western HRP Substrate (GTX14698).
SAP18 antibody detects SAP18 protein by western blot analysis. K562 whole cell extracts and nuclear extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with SAP18 antibody (GTX130522) at a dilution of 1:1000 and developed with Trident femto Western HRP Substrate (GTX14698).

SAP18 antibody

GTX130522
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetSAP18
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Overview

  • Supplier
    GeneTex
  • Product Name
    SAP18 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.49 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID10284
  • Target name
    SAP18
  • Target description
    Sin3A associated protein 18
  • Target synonyms
    2HOR0202, SAP18P, histone deacetylase complex subunit SAP18, 18 kDa Sin3-associated polypeptide, Sin3A-associated protein, 18kDa, cell growth inhibiting protein 38, cell growth-inhibiting gene 38 protein, epididymis secretory sperm binding protein, histone deacetlyase complex subunit SAP18, sin3-associated polypeptide, 18 kDa, sin3-associated polypeptide, p18
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00422
  • Protein Name
    Histone deacetylase complex subunit SAP18
  • Scientific Description
    Histone acetylation plays a key role in the regulation of eukaryotic gene expression. Histone acetylation and deacetylation are catalyzed by multisubunit complexes. The protein encoded by this gene is a component of the histone deacetylase complex, which includes SIN3, SAP30, HDAC1, HDAC2, RbAp46, RbAp48, and other polypeptides. This protein directly interacts with SIN3 and enhances SIN3-mediated transcriptional repression when tethered to the promoter. A pseudogene has been identified on chromosome 2. [provided by RefSeq]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SAP18 using anti-SAP18 antibody (A06381-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: rat RH35 whole cell lysates, Lane 5: mouse NIN/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAP18 antigen affinity purified polyclonal antibody (Catalog # A06381-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SAP18 at approximately 18 kDa. The expected band size for SAP18 is at 18 kDa.
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