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Anti-SATB1 Antibody

144-05800
RayBiotech
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetSATB1
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-SATB1 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6304
  • Target name
    SATB1
  • Target description
    SATB homeobox 1
  • Target synonyms
    DEFDA; DNA-binding protein SATB1; KTZSL; special AT-rich sequence binding protein 1 (binds to nuclear matrix/scaffold-associating DNA)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ01826
  • Protein Name
    DNA-binding protein SATB1
  • Scientific Description
    SATB1 Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Western blot All lanes: SATB1 antibody at 2ug/ml Lane 1: Mouse brain tissue Lane 2: 293T whole cell lysate Lane 3: Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 86, 90 kDa Observed band size: 86 kDa
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Figure 1. Western blot analysis of SATB1 using anti-SATB1 antibody (A01312-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SATB1 antigen affinity purified polyclonal antibody (Catalog # A01312-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SATB1 at approximately 100KD. The expected band size for SATB1 is at 86KD.
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