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SATB1 antibody detects SATB1 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5 % SDS-PAGE, and blotted with SATB1 antibody (GTX114737) diluted by 1:500
SATB1 antibody detects SATB1 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5 % SDS-PAGE, and blotted with SATB1 antibody (GTX114737) diluted by 1:500
SATB1 antibody detects SATB1 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5 % SDS-PAGE, and blotted with SATB1 antibody (GTX114737) diluted by 1:500

SATB1 antibody

GTX114737
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSATB1
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Overview

  • Supplier
    GeneTex
  • Product Name
    SATB1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:10000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.78 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6304
  • Target name
    SATB1
  • Target description
    SATB homeobox 1
  • Target synonyms
    DEFDA, DHDBV, KTZSL, DNA-binding protein SATB1, special AT-rich sequence binding protein 1 (binds to nuclear matrix/scaffold-associating DNA)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ01826
  • Protein Name
    DNA-binding protein SATB1
  • Scientific Description
    This gene encodes a matrix protein which binds nuclear matrix and scaffold-associating DNAs through a unique nuclear architecture. The protein recruits chromatin-remodeling factors in order to regulate chromatin structure and gene expression. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SATB1 using anti-SATB1 antibody (A01312-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SATB1 antigen affinity purified polyclonal antibody (Catalog # A01312-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SATB1 at approximately 100KD. The expected band size for SATB1 is at 86KD.
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