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Immunohistochemistry analysis in human testis and thymus tissues using Anti-SATB1 antibody. Corresponding SATB1 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human testis and thymus tissues using Anti-SATB1 antibody. Corresponding SATB1 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human testis and thymus tissues using Anti-SATB1 antibody. Corresponding SATB1 RNA-seq data are presented for the same tissues.

Anti-SATB1 Antibody

HPA070534
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSATB1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SATB1
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6304
  • Target name
    SATB1
  • Target description
    SATB homeobox 1
  • Target synonyms
    DEFDA; DNA-binding protein SATB1; KTZSL; special AT-rich sequence binding protein 1 (binds to nuclear matrix/scaffold-associating DNA)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ01826
  • Protein Name
    DNA-binding protein SATB1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Western blot All lanes: SATB1 antibody at 2ug/ml Lane 1: Mouse brain tissue Lane 2: 293T whole cell lysate Lane 3: Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 86, 90 kDa Observed band size: 86 kDa
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Molt-4 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with SATB1 (Ser47) Polyclonal Antibody(bs-3390R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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Immunohistochemical staining of human thymus shows strong nuclear positivity in cortical cells.
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