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Anti-SATB2 Antibody

144-64612
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetSATB2
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-SATB2 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Gene ID23314
  • Target name
    SATB2
  • Target description
    SATB homeobox 2
  • Target synonyms
    C2DELq32q33, DEL2Q32Q33, GLSS, DNA-binding protein SATB2, SATB family member 2, SATB2 fusion, special AT-rich sequence-binding protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UPW6
  • Protein Name
    DNA-binding protein SATB2
  • Scientific Description
    SATB2 pAb
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SATB2 using anti-SATB2 antibody (A02588-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SATB2 antigen affinity purified polyclonal antibody (Catalog # A02588-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SATB2 at approximately 90 kDa. The expected band size for SATB2 is at 83 kDa.
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