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Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in glandular cells.

Anti-SEMA3D Antibody

HPA037522
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetSEMA3D
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SEMA3D
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID223117
  • Target name
    SEMA3D
  • Target description
    semaphorin 3D
  • Target synonyms
    coll-2; collapsin 2; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D; semaphorin-3D; Sema-Z2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95025
  • Protein Name
    Semaphorin-3D
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SEMA3D using anti-SEMA3D antibody (A09239-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human U20S whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEMA3D antigen affinity purified polyclonal antibody (Catalog # A09239-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEMA3D at approximately 90 kDa. The expected band size for SEMA3D is at 88,90 kDa.
Product group Antibodies
Boster Bio
Anti-SEMA3D Antibody Picoband(r)A09239-1-CARRIER-FREE
ApplicationsWestern Blot, ELISA
TargetSEMA3D
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Atlas Antibodies
PrEST Antigen SEMA3DAPREST77734
Protein IDO95025
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Anti-SEMA3D Antibody
Product group Antibodies
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Anti-SEMA3D AntibodyA45529
ApplicationsImmunoHistoChemistry
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WB analysis of THP-1 cells using GTX45262 SEMA3D antibody at 0.2-1microg/ml.
Product group Antibodies
GeneTex
SEMA3D antibody, InternalGTX45262
ApplicationsWestern Blot
TargetSEMA3D
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
SEMA3D / Semaphorin 3D AntibodyLS-C402780
ApplicationsELISA, ImmunoHistoChemistry
TargetSEMA3D
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The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using CSB-PA065596(SEMA3D Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200)
Product group Antibodies
Cusabio
SEMA3D AntibodyCSB-PA065596
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetSEMA3D
  • SizePrice