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The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using CSB-PA065596(SEMA3D Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using CSB-PA065596(SEMA3D Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using CSB-PA065596(SEMA3D Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200)

SEMA3D Antibody

CSB-PA065596
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetSEMA3D
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Overview

  • Supplier
    Cusabio
  • Product Name
    SEMA3D Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID223117
  • Target name
    SEMA3D
  • Target description
    semaphorin 3D
  • Target synonyms
    Sema-Z2, coll-2, semaphorin-3D, collapsin 2, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95025
  • Protein Name
    Semaphorin-3D
  • Scientific Description
    This gene Belongs to the semaphorin family. Induces the collapse and paralysis of neuronal growth cones. Could potentially act as repulsive cues toward specific neuronal populations. Strong binding to neuropilin is mediated by the carboxy third of the protein.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SEMA3D using anti-SEMA3D antibody (A09239-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human U20S whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEMA3D antigen affinity purified polyclonal antibody (Catalog # A09239-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEMA3D at approximately 90 kDa. The expected band size for SEMA3D is at 88,90 kDa.
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