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WB analysis of THP-1 cells using GTX45262 SEMA3D antibody at 0.2-1microg/ml.
WB analysis of THP-1 cells using GTX45262 SEMA3D antibody at 0.2-1microg/ml.
WB analysis of THP-1 cells using GTX45262 SEMA3D antibody at 0.2-1microg/ml.

SEMA3D antibody, Internal

GTX45262
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetSEMA3D
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Overview

  • Supplier
    GeneTex
  • Product Name
    SEMA3D antibody, Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.2-2.5 ug/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID223117
  • Target name
    SEMA3D
  • Target description
    semaphorin 3D
  • Target synonyms
    coll-2; collapsin 2; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D; semaphorin-3D; Sema-Z2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95025
  • Protein Name
    Semaphorin-3D
  • Scientific Description
    This gene encodes a member of the semaphorin III family of secreted signaling proteins that are involved in axon guidance during neuronal development. The encoded protein contains an N-terminal Sema domain, an immunoglobulin like domain and a C-terminal basic domain. The protein encoded by this gene binds neuropilin and plays an important role in cardiovascular development. [provided by RefSeq, Aug 2016]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SEMA3D using anti-SEMA3D antibody (A09239-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human U20S whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEMA3D antigen affinity purified polyclonal antibody (Catalog # A09239-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEMA3D at approximately 90 kDa. The expected band size for SEMA3D is at 88,90 kDa.
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