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Anti-SEMA6A Antibody

Anti-SEMA6A Antibody

A45603
Antibodies.com
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
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Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-SEMA6A Antibody
  • Delivery Days Customer
    7
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.2 mg/ml
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Scientific Description
    Rabbit polyclonal antibody to SEMA6A
  • Reactivity
    Human, Mouse
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Sema6A using anti-Sema6A antibody (A06967-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human HELA whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: human U20S whole cell lysates, Lane 5: human Jurkat whole cell lysates, Lane 6: human CACO-2 whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: rat PC-12 whole cell lysates, Lane 9: mouse brain tissue lysates, Lane 10: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Sema6A antigen affinity purified polyclonal antibody (Catalog # A06967-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Sema6A at approximately 114KD. The expected band size for Sema6A is at 114KD.
Product group Antibodies
Boster Bio
Anti-Sema6A Antibody Picoband(r)A06967-1-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSEMA6A
  • SizePrice
Immunohistochemical staining of human small intestine shows strong granular positivity in glandular cells.
Product group Antibodies
Atlas Antibodies
Anti-SEMA6A AntibodyHPA031265
ApplicationsImmunoHistoChemistry
ReactivityHuman
TargetSEMA6A
  • SizePrice
Gel: 6%SDS-PAGE,Lysate: 40 ug,Lane 1-3: 293T cells, Lovo cells, HepG2 cells,Primary antibody: CSB-PA049485(SEMA6A Antibody) at dilution 1/200 dilution,Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,Exposure time: 2 minutes
Product group Antibodies
Cusabio
SEMA6A AntibodyCSB-PA049485
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse
TargetSEMA6A
  • SizePrice
Product group Antibodies
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SEMA6A / Semaphorin 6A Antibody (Biotin)LS-C501860
ApplicationsELISA
ReactivityHuman
TargetSEMA6A
  • SizePrice
Product group Antibodies
Biomatik
Sema6A Polyclonal AntibodyCAC11058
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
TargetSEMA6A
  • SizePrice
Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SEMA6A antibody [HL2120] (GTX638092) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Product group Antibodies
GeneTex
SEMA6A antibody [HL2120]GTX638092
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetSEMA6A
  • SizePrice
Product group Antibodies
Bioss
Sema6A Polyclonal AntibodyBS-2701R
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Human, Mouse, Porcine, Rabbit, Rat
TargetSEMA6A
  • SizePrice