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ProductsBack/SEMA6A antibody [HL2120]
Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SEMA6A antibody [HL2120] (GTX638092) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SEMA6A antibody [HL2120] (GTX638092) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SEMA6A antibody [HL2120] (GTX638092) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

SEMA6A antibody [HL2120]

GTX638092
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSEMA6A
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Overview

  • Supplier
    GeneTex
  • Product Name
    SEMA6A antibody [HL2120]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    HL2120
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID57556
  • Target name
    SEMA6A
  • Target description
    semaphorin 6A
  • Target synonyms
    HT018, SEMA, SEMA6A1, SEMAQ, VIA, semaphorin-6A, SEMA6A-1, sema VIa, sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A, semaphorin 6A-1, semaphorin VIA
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H2E6
  • Protein Name
    Semaphorin-6A
  • Scientific Description
    The transmembrane semaphorin SEMA6A is expressed in developing neural tissue and is required for proper development of the thalamocortical projection (Leighton et al., 2001 [PubMed 11242070]).[supplied by OMIM, Feb 2011]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Sema6A using anti-Sema6A antibody (A06967-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human HELA whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: human U20S whole cell lysates, Lane 5: human Jurkat whole cell lysates, Lane 6: human CACO-2 whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: rat PC-12 whole cell lysates, Lane 9: mouse brain tissue lysates, Lane 10: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Sema6A antigen affinity purified polyclonal antibody (Catalog # A06967-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Sema6A at approximately 114KD. The expected band size for Sema6A is at 114KD.
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