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Immunohistochemical staining of human small intestine shows strong granular positivity in glandular cells.
Immunohistochemical staining of human small intestine shows strong granular positivity in glandular cells.
Immunohistochemical staining of human small intestine shows strong granular positivity in glandular cells.

Anti-SEMA6A Antibody

HPA031265
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSEMA6A
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SEMA6A Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID57556
  • Target name
    SEMA6A
  • Target description
    semaphorin 6A
  • Target synonyms
    HT018, SEMA, SEMA6A1, SEMAQ, VIA, semaphorin-6A, SEMA6A-1, sema VIa, sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A, semaphorin 6A-1, semaphorin VIA
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H2E6
  • Protein Name
    Semaphorin-6A
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Sema6A using anti-Sema6A antibody (A06967-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human HELA whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: human U20S whole cell lysates, Lane 5: human Jurkat whole cell lysates, Lane 6: human CACO-2 whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: rat PC-12 whole cell lysates, Lane 9: mouse brain tissue lysates, Lane 10: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Sema6A antigen affinity purified polyclonal antibody (Catalog # A06967-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Sema6A at approximately 114KD. The expected band size for Sema6A is at 114KD.
Product group Antibodies
Boster Bio
Anti-Sema6A Antibody Picoband(r)A06967-1-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSEMA6A
  • SizePrice
Atlas Antibodies
PrEST Antigen SEMA6AAPREST78454
Protein IDQ9H2E6
  • SizePrice
Anti-SEMA6A Antibody
Product group Antibodies
Antibodies.com
Anti-SEMA6A AntibodyA45603
ApplicationsImmunoHistoChemistry
ReactivityHuman, Mouse
  • SizePrice
Gel: 6%SDS-PAGE,Lysate: 40 ug,Lane 1-3: 293T cells, Lovo cells, HepG2 cells,Primary antibody: CSB-PA049485(SEMA6A Antibody) at dilution 1/200 dilution,Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,Exposure time: 2 minutes
Product group Antibodies
Cusabio
SEMA6A AntibodyCSB-PA049485
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse
TargetSEMA6A
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Product group Antibodies
LifeSpan BioSciences
SEMA6A / Semaphorin 6A Antibody (Biotin)LS-C501860
ApplicationsELISA
ReactivityHuman
TargetSEMA6A
  • SizePrice
Product group Antibodies
Biomatik
Sema6A Polyclonal AntibodyCAC11058
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
TargetSEMA6A
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Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SEMA6A antibody [HL2120] (GTX638092) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Product group Antibodies
GeneTex
SEMA6A antibody [HL2120]GTX638092
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetSEMA6A
  • SizePrice
Product group Antibodies
Bioss
Sema6A Polyclonal AntibodyBS-2701R
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Human, Mouse, Porcine, Rabbit, Rat
TargetSEMA6A
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