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Gel: 6%SDS-PAGE,Lysate: 40 ug,Lane 1-3: 293T cells, Lovo cells, HepG2 cells,Primary antibody: CSB-PA049485(SEMA6A Antibody) at dilution 1/200 dilution,Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,Exposure time: 2 minutes
Gel: 6%SDS-PAGE,Lysate: 40 ug,Lane 1-3: 293T cells, Lovo cells, HepG2 cells,Primary antibody: CSB-PA049485(SEMA6A Antibody) at dilution 1/200 dilution,Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,Exposure time: 2 minutes
Gel: 6%SDS-PAGE,Lysate: 40 ug,Lane 1-3: 293T cells, Lovo cells, HepG2 cells,Primary antibody: CSB-PA049485(SEMA6A Antibody) at dilution 1/200 dilution,Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,Exposure time: 2 minutes

SEMA6A Antibody

CSB-PA049485
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetSEMA6A
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Overview

  • Supplier
    Cusabio
  • Product Name
    SEMA6A Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID57556
  • Target name
    SEMA6A
  • Target description
    semaphorin 6A
  • Target synonyms
    HT018; SEMA; sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A; sema VIa; SEMA6A1; SEMA6A-1; semaphorin 6A-1; semaphorin VIA; semaphorin-6A; SEMAQ; VIA
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H2E6
  • Protein Name
    Semaphorin-6A
  • Scientific Description
    The transmembrane semaphorin SEMA6A is expressed in developing neural tissue and is required for proper development of the thalamocortical projection. Cell surface receptor for PLXNA2 that plays an important role in cell-cell signaling. Required for normal granule cell migration in the developing cerebellum. Promotes reorganization of the actin cytoskeleton and plays an important role in axon guidance in the developing central nervous system.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Sema6A using anti-Sema6A antibody (A06967-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human HELA whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: human U20S whole cell lysates, Lane 5: human Jurkat whole cell lysates, Lane 6: human CACO-2 whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: rat PC-12 whole cell lysates, Lane 9: mouse brain tissue lysates, Lane 10: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Sema6A antigen affinity purified polyclonal antibody (Catalog # A06967-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Sema6A at approximately 114KD. The expected band size for Sema6A is at 114KD.
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