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Immunohistochemical staining of human thyroid gland shows strong nuclear positivity in glandular cells.
Immunohistochemical staining of human thyroid gland shows strong nuclear positivity in glandular cells.
Immunohistochemical staining of human thyroid gland shows strong nuclear positivity in glandular cells.

Anti-SGTA Antibody

HPA056309
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetSGTA
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SGTA
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6449
  • Target name
    SGTA
  • Target description
    small glutamine rich tetratricopeptide repeat co-chaperone alpha
  • Target synonyms
    alphaSGT; alpha-SGT; hSGT; protein containing three tetratricopeptide repeats; SGT; SGT1; small glutamine rich tetratricopeptide repeat containing alpha; small glutamine-rich tetratricopeptide repeat (TPR)-containing, alpha; small glutamine-rich tetratricopeptide repeat-containing protein alpha; UBP; viral protein U-binding protein; Vpu; vpu-binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43765
  • Protein Name
    Small glutamine-rich tetratricopeptide repeat-containing protein alpha
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SGTA using anti-SGTA antibody (A03818-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SGTA antigen affinity purified polyclonal antibody (Catalog # A03818-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SGTA at approximately 37 kDa. The expected band size for SGTA is at 34 kDa.
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