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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA021204ESR2HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA021204ESR2HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA021204ESR2HU at dilution of 1:100

SGTA Antibody

CSB-PA021204ESR2HU
Cusabio
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSGTA
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Overview

  • Supplier
    Cusabio
  • Product Name
    SGTA Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6449
  • Target name
    SGTA
  • Target description
    small glutamine rich tetratricopeptide repeat co-chaperone alpha
  • Target synonyms
    alphaSGT; alpha-SGT; hSGT; protein containing three tetratricopeptide repeats; SGT; SGT1; small glutamine rich tetratricopeptide repeat containing alpha; small glutamine-rich tetratricopeptide repeat (TPR)-containing, alpha; small glutamine-rich tetratricopeptide repeat-containing protein alpha; UBP; viral protein U-binding protein; Vpu; vpu-binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43765
  • Protein Name
    Small glutamine-rich tetratricopeptide repeat-containing protein alpha
  • Scientific Description
    Co-chaperone that binds directly to HSC70 and HSP70 and regulates their ATPase activity.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SGTA using anti-SGTA antibody (A03818-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SGTA antigen affinity purified polyclonal antibody (Catalog # A03818-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SGTA at approximately 37 kDa. The expected band size for SGTA is at 34 kDa.
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