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ProductsBack/Goat anti-Sgta (mouse aa84-97)

Goat anti-Sgta (mouse aa84-97)

EB11841
Everest Biotech
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetSGTA
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-Sgta (mouse aa84-97) Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID6449
  • Target name
    SGTA
  • Target description
    small glutamine rich tetratricopeptide repeat co-chaperone alpha
  • Target synonyms
    SGT, SGT1, Vpu, alphaSGT, hSGT, small glutamine-rich tetratricopeptide repeat-containing protein alpha, UBP, alpha-SGT, protein containing three tetratricopeptide repeats, small glutamine rich tetratricopeptide repeat containing alpha, small glutamine-rich tetratricopeptide repeat (TPR)-containing, alpha, viral protein U-binding protein, vpu-binding protein
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_078775.1. GeneIDs all Nonhuman: 52551 (mouse); 64667 (rat);. Purification: Antigen affinity purified. Names and symbols: Sgta; small glutamine-rich tetratricopeptide repeat (TPR)-containing, alpha; 5330427H01Rik; AI194281; D10Ertd190e; Sgt; Stg; alpha-SGT; small glutamine-rich tetratricopeptide repeat (TPR) containing protein; small glutamine-rich tetratricopeptide repeat-c
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Pig, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SGTA using anti-SGTA antibody (A03818-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SGTA antigen affinity purified polyclonal antibody (Catalog # A03818-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SGTA at approximately 37 kDa. The expected band size for SGTA is at 34 kDa.
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