More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Anti-SPI1 Antibody

Anti-SPI1 Antibody

102-20308
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetSPI1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-SPI1 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1835CT291.10.52
  • Gene ID6688
  • Target name
    SPI1
  • Target description
    Spi-1 proto-oncogene
  • Target synonyms
    AGM10, OF, PU.1, SFPI1, SPI-1, SPI-A, transcription factor PU.1, 31 kDa transforming protein, hematopoietic transcription factor PU.1, spleen focus forming virus (SFFV) proviral integration oncogene spi1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP17947
  • Protein Name
    Transcription factor PU.1
  • Scientific Description
    Mouse Anti-SPI1 Antibody, 200 microl
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

Go to our frequently asked questions

Related products

Hela cell lysates probed with PU.1/Spi1 Polyclonal Antibody, Unconjugated (bs-19594R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Product group Antibodies
Bioss
PU.1/Spi1 Polyclonal AntibodyBS-19594R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Equine, Human, Mouse, Porcine, Rat, Sheep
TargetSPI1
  • SizePrice
Western Blot analysis of HepG2 cells using PU.1 Polyclonal Antibody
Product group Antibodies
Cusabio
SPI1 AntibodyCSB-PA003887
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Monkey, Mouse, Rat
TargetSPI1
  • SizePrice
Anti-SPI1 Antibody
Product group Antibodies
Antibodies.com
Anti-SPI1 AntibodyA97901
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
SPI1 / PU.1 AntibodyLS-C831700
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetSPI1
  • SizePrice
Immunohistochemistry analysis in human bone marrow and pancreas tissues using HPA044653 antibody. Corresponding SPI1 RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
Anti-SPI1 AntibodyHPA044653
ApplicationsChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetSPI1
  • SizePrice
Product group Antibodies
Everest Biotech
Goat anti-PU.1 AntibodyEB08429
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Rat
TargetSPI1
  • SizePrice
Product group Antibodies
MedChem Express
PU.1/SPI1 Antibody (YA118, Rabbit)HY-P80287
TargetSPI1
  • SizePrice
THP-1 whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PU.1 antibody [N1C1] (GTX101581) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
References
GeneTex
PU.1 antibody [N1C1]GTX101581
ApplicationsWestern Blot
ReactivityHuman, Mouse
TargetSPI1
  • SizePrice
Figure 1. Western blot analysis of PU.1/SPI1 using anti-PU.1/SPI1 antibody (A01116-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PU.1/SPI1 antigen affinity purified polyclonal antibody (Catalog # A01116-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PU.1/SPI1 at approximately 40 kDa. The expected band size for PU.1/SPI1 is at 40 kDa.
Product group Antibodies
Boster Bio
Anti-PU.1/SPI1 Antibody Picoband(r)A01116-1-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSPI1
  • SizePrice