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Western Blot analysis of HepG2 cells using PU.1 Polyclonal Antibody
Western Blot analysis of HepG2 cells using PU.1 Polyclonal Antibody
Western Blot analysis of HepG2 cells using PU.1 Polyclonal Antibody

SPI1 Antibody

CSB-PA003887
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Monkey, Mouse, Rat
TargetSPI1
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Overview

  • Supplier
    Cusabio
  • Product Name
    SPI1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6688
  • Target name
    SPI1
  • Target description
    Spi-1 proto-oncogene
  • Target synonyms
    31 kDa transforming protein; hematopoietic transcription factor PU.1; OF; PU.1; SFPI1; SPI-1; SPI-A; spleen focus forming virus (SFFV) proviral integration oncogene spi1; transcription factor PU.1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP17947
  • Protein Name
    Transcription factor PU.1
  • Reactivity
    Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PU.1/SPI1 using anti-PU.1/SPI1 antibody (A01116-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PU.1/SPI1 antigen affinity purified polyclonal antibody (Catalog # A01116-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PU.1/SPI1 at approximately 40 kDa. The expected band size for PU.1/SPI1 is at 40 kDa.
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