THP-1 whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PU.1 antibody [N1C1] (GTX101581) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
THP-1 whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PU.1 antibody [N1C1] (GTX101581) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
PU.1 antibody [N1C1]
GTX101581
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetSPI1
Overview
- SupplierGeneTex
- Product NamePU.1 antibody [N1C1]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.84 mg/ml
- ConjugateUnconjugated
- Gene ID6688
- Target nameSPI1
- Target descriptionSpi-1 proto-oncogene
- Target synonymsAGM10, OF, PU.1, SFPI1, SPI-1, SPI-A, transcription factor PU.1, 31 kDa transforming protein, hematopoietic transcription factor PU.1, spleen focus forming virus (SFFV) proviral integration oncogene spi1
- HostRabbit
- IsotypeIgG
- Protein IDP17947
- Protein NameTranscription factor PU.1
- Scientific DescriptionThis gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Chou CL, Li CH, Fang TC. Benefits of Valsartan and Amlodipine in Lipolysis through PU.1 Inhibition in Fructose-Induced Adiposity. Nutrients. 2022,14(18). doi: 10.3390/nu14183759Read this paper
Datasheet
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