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Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in cells outside reaction centra.
Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in cells outside reaction centra.
Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in cells outside reaction centra.

Anti-STK4 Antibody

HPA015270
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
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Product group Antibodies
TargetSTK4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-STK4
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6789
  • Target name
    STK4
  • Target description
    serine/threonine kinase 4
  • Target synonyms
    hippo homolog; kinase responsive to stress 2; KRS2; mammalian STE20-like protein kinase 1; mammalian sterile 20-like 1; MST1; serine/threonine-protein kinase 4; serine/threonine-protein kinase Krs-2; STE20-like kinase MST1; YSK3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13043
  • Protein Name
    Serine/threonine-protein kinase 4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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The image on the left is immunohistochemistry of paraffin-embedded Human Lymphoma tissue using CSB-PA168071(STK3/STK4 Antibody) at dilution 1/70, on the right is treated with synthetic peptide. (Original magnification: x200)
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Figure 1. Western blot analysis of MST1/STK4 using anti-MST1/STK4 antibody (A00859-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human Ramos whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MST1/STK4 antigen affinity purified polyclonal antibody (Catalog # A00859-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MST1/STK4 at approximately 56 kDa. The expected band size for MST1/STK4 is at 56,52 kDa.
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Lane 1: Mouse Liver tissue lysates; Lane 2: Mouse Raw264.7 cell lysates; Lane 3: Mouse NIH/3T3 cell lysates; Lane 4: Rat Liver tissue lysates ; Lane 5: Human SH-SY5Y cell lysates ; Lane 6: Human HL60 cell lysates probed with MST1 Polyclonal Antibody, Unconjugated (bs-28134R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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