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The image on the left is immunohistochemistry of paraffin-embedded Human Lymphoma tissue using CSB-PA168071(STK3/STK4 Antibody) at dilution 1/70, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human Lymphoma tissue using CSB-PA168071(STK3/STK4 Antibody) at dilution 1/70, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human Lymphoma tissue using CSB-PA168071(STK3/STK4 Antibody) at dilution 1/70, on the right is treated with synthetic peptide. (Original magnification: x200)

STK4 Antibody

CSB-PA168071
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetSTK4
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Overview

  • Supplier
    Cusabio
  • Product Name
    STK4 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6789
  • Target name
    STK4
  • Target description
    serine/threonine kinase 4
  • Target synonyms
    hippo homolog; kinase responsive to stress 2; KRS2; mammalian STE20-like protein kinase 1; mammalian sterile 20-like 1; MST1; serine/threonine-protein kinase 4; serine/threonine-protein kinase Krs-2; STE20-like kinase MST1; YSK3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13043
  • Protein Name
    Serine/threonine-protein kinase 4
  • Scientific Description
    Sterile-20 (Ste20) is a serine/threonine kinase in Saccharomyces cerevisiae that is involved in relaying signals from G protein-coupled receptors to cyto-solic MAP kinase cascades. Mammalian protein kinases that display sequence similarity to Ste20 are divided into two groups, the PAK subfamily and the GCK subfamily. The mammalian Ste20-like kinases (MST kinases), also known as Krs proteins, are members of the GCK subfamily. Ksr-1 (MST-2) and Ksr-2 (MST-1) are both direct substrates of caspase-3 that accelerate caspase-3 activation.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Lane 1: Mouse Liver tissue lysates; Lane 2: Mouse Raw264.7 cell lysates; Lane 3: Mouse NIH/3T3 cell lysates; Lane 4: Rat Liver tissue lysates ; Lane 5: Human SH-SY5Y cell lysates ; Lane 6: Human HL60 cell lysates probed with MST1 Polyclonal Antibody, Unconjugated (bs-28134R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of MST1/STK4 using anti-MST1/STK4 antibody (A00859-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human Ramos whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MST1/STK4 antigen affinity purified polyclonal antibody (Catalog # A00859-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MST1/STK4 at approximately 56 kDa. The expected band size for MST1/STK4 is at 56,52 kDa.
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