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Anti-MST1/STK4 Antibody

CAB8043
Assay Genie
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetSTK4
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Overview

  • Supplier
    Assay Genie
  • Product Name
    Anti-MST1/STK4 Antibody
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB,IHC,IF,IP
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    Not applicable
  • Conjugate
    Unconjugated
  • Gene ID6789
  • Target name
    STK4
  • Target description
    serine/threonine kinase 4
  • Target synonyms
    KRS2, MST1, YSK3, serine/threonine-protein kinase 4, STE20-like kinase MST1, hippo homolog, kinase responsive to stress 2, mammalian STE20-like protein kinase 1, mammalian sterile 20-like 1, serine/threonine-protein kinase Krs-2
  • Host
    Rabbit
  • Protein IDQ13043
  • Protein Name
    Serine/threonine-protein kinase 4
  • Scientific Description
    MST1/STK4 Antibody is a premium polyclonal that offers outstanding performance and reliability for demanding research applications. Rigorously validated for WB, IHC-P, IF/ICC, IP, ELISA, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Human,Mouse,Rat samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 100microL format to meet your experimental needs. For optimal performance, store at -20°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity
    Human
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MST1/STK4 using anti-MST1/STK4 antibody (A00859-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human Ramos whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MST1/STK4 antigen affinity purified polyclonal antibody (Catalog # A00859-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MST1/STK4 at approximately 56 kDa. The expected band size for MST1/STK4 is at 56,52 kDa.
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ReactivityHuman, Mouse, Rat
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Lane 1: Mouse Liver tissue lysates; Lane 2: Mouse Raw264.7 cell lysates; Lane 3: Mouse NIH/3T3 cell lysates; Lane 4: Rat Liver tissue lysates ; Lane 5: Human SH-SY5Y cell lysates ; Lane 6: Human HL60 cell lysates probed with MST1 Polyclonal Antibody, Unconjugated (bs-28134R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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The image on the left is immunohistochemistry of paraffin-embedded Human Lymphoma tissue using CSB-PA168071(STK3/STK4 Antibody) at dilution 1/70, on the right is treated with synthetic peptide. (Original magnification: x200)
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Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using STK4(GTX109294) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
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Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in cells outside reaction centra.
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